PRODUCTION OF AGENTS FOR BIOCONTROL OF APPLE FUSARIUM ROT BY SOILBORNE STREPTOMYCETES

Received: 16th June 2021; Revised: 2nd November 2021, 10th January 2022, 13th January 2022; Accepted: 17th January 2022

Authors

  • Ivana Mitrović University of Novi Sad, Faculty of Technology Novi Sad, Bulevar Cara Lazara 1, Novi Sad, Serbia
  • Jovana Grahovac University of Novi Sad, Faculty of Agriculture, Trg Dositeja Obradovića 8, Novi Sad, Serbia
  • Jelena Dodić University of Novi Sad, Faculty of Technology Novi Sad, Bulevar Cara Lazara 1, Novi Sad, Serbia
  • Mila Grahovac University of Novi Sad, Faculty of Agriculture,Trg Dositeja Obradovića 8, Novi Sad, Serbia
  • Zorana Trivunović University of Novi Sad, Faculty of Technology Novi Sad, Bulevar Cara Lazara 1, Novi Sad, Serbia
  • Siniša Dodi University of Novi Sad, Faculty of Technology Novi Sad, Bulevar Cara Lazara 1, Novi Sad, Serbia

DOI:

https://doi.org/10.20319/mijst.2022.73.5768

Keywords:

Biocontrol, Bioprocess, Fusarium Avenaceum, Streptomyces Hygroscopicus

Abstract

Within this paper, the potential of Streptomyces hygroscopicus in biocontrol of two isolates of Fusarium avenaceum isolated from apple fruits in the storage was tested. Production of S. hygroscopicus biocontrol agent was performed in 3 l stirred tank bioreactor at an aeration rate of 1.5 vvm and mixing speed of 150 rpm. Bioprocess was realized at 26 ±1°C for 168 hours. The activity of bioagents production was tested using in vitro diffusion method with wells. Also, residual glycerol, residual nitrogen content, and cell biomass were determined to analyze bioprocess parameters. The results showed that the highest production of bioagents was observed in 96h of cultivation when the largest zone diameters were formed against F. avenaceum KA12 and KA13 of 40.67±1.15 and 43.33±0.58 mm, respectively. The activity of the produced bioagents was confirmed in in planta research, where the difference between necrosis diameter of treated and control samples were 2.79 (for KA12) and 2.17 (for KA13) times smaller.

References

Adedokun, O.O., Ojewola, G.S. & Ahamefule, F.O. (2018). Preliminary investigation of the nutritive potential of umucass 36 cassava root meal as substitute for maize in broiler diets. International Journal of Science and Technology, 4(3), 79-86. https://doi.org/10.20319/mijst.2018.43.7986

Bjelić, D., Ignjatov, M., Marinković, J., Milošević, D., Nikolić, Z., Gvozdanović-Varga, J. & Karaman, M. (2018). Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic. Zemdirbyste-Agriculture, 105 (4), 369–376.

https://doi.org/10.13080/z-a.2018.105.047

EPA (1979): EPA Manual 351.2. Determination of total Kjeldahl nitrogen by semi-automated colorimetry. 1979. Report No. EPA 60014-79-020

Gashgari, R., Ameen, F., Al-Homaidi, E., Gherbawy, Y., Al Nadhari, S. & Vijayan, V. (2019). Mycotoxigenic fungi contaminating wheat; toxicity of different Alternaria compacta strains. Saudi Journal of Biological Science, 26, 210–215. https://doi.org/10.1016/j.sjbs.2018.10.007

Grahovac, J., Mitrović, I., Dodić, J., Grahovac, M., Rončević, Z., Dodić, S. & Jokić, A. (2020). Biocontrol agent for apple Fusarium rot: optimization of production by Streptomyces hygroscopicus. Zemdirbyste-Agriculture, 107 (3), 263-270. https://doi.org/10.13080/z-a.2020.107.034

Juhnevica-Radenkova, K., Radenkova, V. & Seglina, D. (2016). Influence of 1-MCP treatment and storage conditions on the development of microorganisms on the surface of apples grown in Latvia. Zemdirbyste-Agriculture, 103 (2), 215–220. https://doi.org/10.13080/z-a.2016.103.028

Kanini, G., Katsifas, E., Savvides, A. & Karagouni, A. (2013). Streptomyces rochei ACTA1551, an indigenous Greek isolate studied as a potential biocontrol agent against Fusarium oxysporum .sp. lycopersici. BioMed Research International, 1-10. https://doi.org/10.1155/2013/387230

Meanwell, R.J.L. & Shama, G. (2008). Production of streptomycin from chitin using Streptomyces griseus in bioreactors of different configurations. Bioresours Technology, 99, 5634-5639. https://doi.org/10.1016/j.biortech.2007.10.036

Mitrović, I., Grahovac, J., Hrustić, J., Jokić, A., Dodić, J., Mihajlović, M. & Grahovac, M. (2021). Utilization of waste glycerol for the production of biocontrol agents nigericin and niphimycin by Streptomyces hygroscopicus: bioprocess development. Environmental Technology, https://doi.org/10.1080/09593330.2021.1913241

Mojićević, M, Grahovac, J, Petković, M, Vučković, I., Dodić, J., Dodić, S. & Vojnović, S. (2017). Production of nigericin and niphimycin by soil isolate Streptomyces sp. MS1: anti-candida bioassay guided response surface methodology for the optimized culture medium. Facta Universitatis, Series Physics, Chemistry and Technology, 15 (1), 1-16. https://doi.org/10.2298/FUPCT1701001M

Olanrewaju, O.S. & Babalola, O.O. (2019). Streptomyces: implications and interactions in plant growth promotion. Applied Microbiology and Biotechnology, 103(3): 1179-1188. https://doi.org/10.1007/s00253-018-09577-y

Önder, U. & Kamil, E. (2019). Design of mobile and functional photobioreactor. International Journal of Science and Technology, 4(3), 150-156. https://doi.org/10.20319/mijst.2019.43.150156

Sadhasivam, S., Shanmugam, P. & Yun, K. (2010). Biosynthesis of silver nanoparticles by Streptomyces hygroscopicus and antimicrobial activity against medically important pathogenic microorganisms. Colloids and Surfaces B: Biointerfaces, 81, 358-362. https://doi.org/10.1016/j.colsurfb.2010.07.036

Sever, Z., Ivić, D., Kos, T. & Miličević, T. (2012). Identification of Fusarium species isolated from stored apple fruit in Croatia. Archives of Industrial Hygiene and Toxicology, 63 (4), 463–470. https://doi.org/10.2478/10004-1254-63-2012-2227

Singh, L.S., Mazumder, S. & Bora, T.C. (2009). Optimisation of process parameters for growth and bioactive metabolite produced by a salt-tolerant and alkaliphilic actinomycete, Streptomyces tanashiensis strain A2D. Journal of Medical Mycology, 19, 225-233. https://doi.org/10.1016/j.mycmed.2009.07.006

Singh, N. & Rai, V. (2012). Optimization of cultural parameters for antifungal and antibacterial metabolite from microbial isolate; Streptomyces rimosus MTCC 10792 from soil of Chhattisgarh. International Journal of Pharmacy and Pharmaceutical Sciences, 4, 94-101.

Sousa, M.F.V.Q., Lopes, C.E. & Pereir N. (2002). Development of a bioprocess for the production of actinomycin-D. Brazilian Journal of Chemical Engineering, 19(3), 277-285. https://doi.org/10.1590/S0104-66322002000300002

Tadijan, I., Grahovac, J., Dodić, J., Grahovac, M. & Dodić, S. (2016). Effect of cultivation time on production of antifungal metabolite(s) by Streptomyces hygroscopicus in laboratory-scale bioreactor. Journal of Phytopathology, 164, 310-317. https://doi.org/10.1111/jph.12458

Wenneker, M., Pham, K. T. K., Lemmers, M. E. C., de Boer, F. A., van der Lans, A. M., van Leeuwen, P. J., Hollinger, T. C. & Thomma, B. P. H. J. (2016). First report of Fusarium avenaceum causing wet core rot of ‘Elstar’ apples in the Netherlands. Plant Disease, 100 (7), 1501. https://doi.org/10.1094/PDIS-01-16-0034-PDN

Yen, H.W. & Li, Y.L. (2014). The effects of viscosity and aeration rate on rapamycin production in an airlift bioreactor by using Streptomyces hygroscopicus. Journal of the Taiwan Institute of Chemical Engineers, 45, 1149-1153. https://doi.org/10.1016/j.jtice.2014.01.014

Downloads

Published

2022-01-19

How to Cite

Mitrović, I., Grahovac, J., Dodić, J., Grahovac, M., Trivunović, Z., & Dodić, S. (2022). PRODUCTION OF AGENTS FOR BIOCONTROL OF APPLE FUSARIUM ROT BY SOILBORNE STREPTOMYCETES: Received: 16th June 2021; Revised: 2nd November 2021, 10th January 2022, 13th January 2022; Accepted: 17th January 2022. MATTER: International Journal of Science and Technology, 7(3), 57–68. https://doi.org/10.20319/mijst.2022.73.5768

Issue

Vol. 7 No. 3 (2021): Regular Issue

Section

Articles

License

Copyright of Published Articles

Author(s) retain the article copyright and publishing rights without any restrictions.

Creative Commons License
All published work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.