PREVALANCE OF CANDIDIASIS IN CHILDREN IN MUMBAI
Keywords:Candida Albicans, Fluconazole, Amphotericin B
This study aims to determine the prevalence of infection of Candida species and its resistance to anti-fungal agents amongst children in Mumbai. Total 169 clinical isolates of Candida species from various clinical specimens namely blood, urine, stool; Cerebral spinal fluid, liver abscess,Endotracheal secretion and ventric tip were included in the study. They were screened and identified by staining, culture and microscopy, followed by isolation on specialized chromogenic agar. Antifungal susceptibility tests of these isolates were carried out against Amphotericin B (50mcg), Itraconazole (10mcg), Clotrimazole (10mcg), Ketoconazole (30mcg), Miconazole (30mcg), and Fluconazole (10mcg) and were reported based on CLSI guidelines (2008).The prevalence of Candida infections was found to be greater in males (69%) as compared to female (31%) children. In the age group of 0-5 years, the occurrence of Candida infection was substantially higher as compared to the others age group. Candida albicans was found to be the major causative agent of fungal infections. The susceptibility of isolates to antifungal agents was found to differ in male and female patients. Overall, highest resistance was observed to Fluconazole (27.1%) while, lowest resistance to Amphotericin B (28.7%). Males exhibited highest resistance to Fluconazole (27.1%) while, females showed highest resistance to Amphotericin B (28.7%). Higher resistance was found to the azole group of antifungal agents. Hence, Amphotericin B can be the choice of treatment of Candida infection in children followed by Miconazole.
Bandi, A., Kumar, R., Nirkhiwale, S., & Pahwa, N. (2014). Species distribution and drug susceptibility of candida in clinical isolates from a tertiary care centre at Indore. Indian Journal of Medical Microbiology, 32, 44-48.
Bhagat, B. P . (2014). Vulvovaginal Candidiasis: Isolation and Identification of Candida from Reproductive Age group Woman Res. J. Recent. Sci. , 3, 30-33
Klevay, M., Ebinger, A., Diekema, D., Messer, S., Hollis, R., & Pfaller, M. (2005). Disk Diffusion Testing Using Candida sp. Colonies Taken Directly from CHROMagar Candida Medium May Decrease Time Required To Obtain Results. Journal of Clinical Microbiology, 43(7), 3497-3499. doi: 10.1128/jcm.43.7.3497-3499.2005
Kothari A, S. V. (2008). Epidemiology of candida bloodstream infections in a tertiary care institute in India. Indian J Med Microbiol, 27, 171-172.
Lozano-Chiu, M., Nelson, P. W., Paetznick, V. L., & Rex, J. H. (1999). Disk Diffusion Method for Determining Susceptibilities of Candida spp. to MK-0991. Journal of Clinical Microbiology, 37(5), 1625-1627.
Mohandas, B., & Ballal, M. (2011). Distribution of Candida species in different clinical samples and their Virulence formation, Proteinase and Phospholipase production: A study on hospitalized patients in Southern India. Jouranl of Global Infectious Disease, 3(1), 4-8.
National Committee for Clinical Laboratory Standards. (2008). Method for antifungal disk diffusion susceptibility testing of yeasts. Approved Sandard M44-A2
Patel, L. R., Pethani, J. D., Bhatia, P., Rathod, S. D., & Shah, P. D. (2012). Prevalence of Candida infections and its anti-fungal susceptibility pattern in tertiary care hospital, Ahmedabad. National Journal of Medical Research, 2(4), 439-441.
Pfaller, M. A., & Diekema, D. J. (2007). Epidemiology of invasive candidiasis: a persistent public health problem. Clinical microbiology reviews, 20(1), 133-163.
Sardi, J. C. O., Scorzoni, L., Bernardi, T., Fusco-Almeida, A. M., & Giannini, M. J. S. M. (2013). Candida species: current epidemiology, pathogenicity, biofilm formation, natural antifungal products and new therapeutic options. J Med Microbiol, 62, 10-24.
Sharma, M., & Solanki, A. (2014). Prevalence of Candida infections in pregnant women with and without diabetes. International Journal of Current Microbiology and Applied Sciences, 3(4), 605-610.
Zomorodian, K., Rahimi, M. J., Pakshir, K., Motamedi, M., Ghiasi, M. R., & Rezashah, H. (2011). Determination of antifungal susceptibility patterns among the clinical isolates of Candida species. Journal of global infectious diseases, 3(4), 357
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