THE THERAPEUTIC TREATMENT OF PEPITEM INCREASES THE PROTEIN EXPRESSION OF SIRT1 IN A MOUSE MODEL OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS (EAE) AS A MODEL FOR HUMAN MS

Authors

  • Mohammed Alassiri Department of Basic Sciences, College of Science and Health Professions, King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), King Abdullah International Medical Research Center (KAIMRC). Riyadh, KSA; Department of Pathology and Laboratory Medicine, King Abdulaziz Medical City (KAMC), Ministry of National Guard-Health Affairs (MNGHA), Riyadh

DOI:

https://doi.org/10.20319/icrlsh.2024.1625

Keywords:

PEPITEM, Experimental Autoimmune Encephalomyelitis,, SIRT1, Anti-Inflammatory

Abstract

Objective: To investigate the effect of the prophylactic and therapeutic treatment of the immunopeptide PEPITEM on the protein expression of SIRT1 in a mouse model of experimental autoimmune encephalomyelitis (EAE) as a model for human MS.  

Methods: Using C57BL/6 female mice, we dosed the PEPITEM in the EAE model via intraperitoneal injections either prophylactically or therapeutically. The disease was induced using MOG35-55 and complete Freund's adjuvants augmented with pertussis toxin. The EAE score was recorded daily until the end of the experiment (21 days). A Western blot analysis was performed on the brain lysate to measure the protein concentration of SIRT1.

Results: The therapeutic treatment with PEPITEM increased the protein expressions of SIRT1 on the EAE mice whereas the prophylactic injections did not affect the protein expression of SIRT1.

Conclusion: Collectively, the therapeutic treatment with PEPITEM suggesting anti-inflammatory effect of PEPITEM on the brain damage in EAE mice which offers a novel and safe strategy for drug therapy in MS, opening new avenues for research and treatment.

References

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Published

2024-02-29

How to Cite

Alassiri, M. (2024). THE THERAPEUTIC TREATMENT OF PEPITEM INCREASES THE PROTEIN EXPRESSION OF SIRT1 IN A MOUSE MODEL OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS (EAE) AS A MODEL FOR HUMAN MS. LIFE: International Journal of Health and Life-Sciences, 16–25. https://doi.org/10.20319/icrlsh.2024.1625