ANTIOXIDANT ACTIVITY OF CATECHINS ISOLATE OF UNCARIA GAMBIER ROXB IN MALE RATS

Authors

  • Muhammad Yanis Musdja Department of Pharmacy, Faculty of Health Sciences, State Islamic University, Jakarta, Indonesia
  • Hary Abdul Rahman Department of Pharmacy, Faculty of Health Sciences, State Islamic University, Jakarta, Indonesia
  • Delina Hasan Department of Pharmacy, Faculty of Health Sciences, State Islamic University, Jakarta, Indonesia

DOI:

https://doi.org/10.20319/lijhls.2018.42.3446

Keywords:

Antioxidant, Cancer, Catechins, Medicinal Plant, Natural Product, Uncaria Gambir, Male Rats

Abstract

The aims of this study were to determine the antioxidant activity of catechins isolates of gambir by measuring the levels of Malondialdehid (MDA) in male white rats. Methods: Catechins of gambir were isolated by using partition method with ethyl acetate solvent. Gambir quality was determined based on National Standard of Indonesia: SNI 01-3391-2000. The yield of catechins obtained from the gambir isolate was determined as (+)-catechins  by comparing with standard  (+)-catechins and measured by a spectrophotometer UV-VIS at wavelength 279 nm. A total of 25 male rats were divided into 5 groups. Administration of catechins was suspended in  0.5% Na CMC with dose of 5 mg/kg b.w, 10 mg/kg b.w and 20 mg/kg b.w, as  positive control was used the suspension of vitamin E, 20 mg/kg b.w in Na-CMC 0.5% and as  negative control was used 0.5% Na-CMC suspension. Administration of test preparations was performed per oral, 1 times per day for 7 days. On the eighth day, the rats were made become oxidative stress with swimming in water about 1 hour, until almost drowned.  MDA levels of rats in each group were measured by comparing MDA levels, before were given test preparation on first day and after were given test preparations on the eighth day. The MDA level of serum rats  were measured based on Wills method. Result: The statistical test of catechins isolate of gambir showed that all dosage of catechins isolate test of gambir had antioxidant effect and had significant difference to negative control (p <0.05). Doses 5 and 10 mg/kg b.w did not show significant differences with  vitamin A as positive control, while Dose 20 mg/kg b.w  gave the strongest antioxidant effect and had significant difference with positive control (p <0.05). Conclusion: Catechins of Gambir has stronger potential as an antioxidant than vitamin A. 

References

Amir M, Mujeeb M, Khan A, Ashraf K, Sharma D, Aqil M. (2012, Jan-March ). Phytochemical analysis and in vitro antioxidant activity of Uncaria gambir, Int J. Green Pharm, 67-72., https://www.researchgate.net/publication/273685306_Phytochemical_analysis_and_in_vitro_antioxidant_activity_of_Uncaria_gambir

Amos. (2010). The content of catechins of Gambir Production Centers in Indonesia (In Bahasa : Kandungan Katekin Gambir Sentra Produksi di Indonesia), Jurnal Standardisasi Vol. 12, No. 3, 149 – 155. http://id.portalgaruda.org/index.php?ref=browse&mod=viewarticle&article=200448

Anggraini T, Tai A, Yoshino T, Itani, T. (2011, January). Antioxidative activity and catechin content of four kinds of Uncaria gambir extracts from West Sumatra, Indonesia, African Journal of Biochemistry Research Vol. 5(1), 33-38., http://www.academicjournals.org/article/article1380127174_Tuty%2520et%2520al.

Armoskaite V, Ramanauskiene K, Maruska A, Razukas A, Dagilyte A, Baranauskas A and Briedis V. (2011, March) The analysis of quality and antioxidant activity of green tea extracts, Journal of Medicinal Plants Research Vol. 5(5), 4, 811-816. http://www.academicjournals.org/article/article1380623511_Armoskaite%20et%20al%20Pdf.

Banjarnahor SDS, Artanti N. ( 2014, November). Med. J. Indones, Vol. 23, No. 4, 239-244. DOI: https://doi.org/10.13181/mji.v23i4.1015.

de Zwart LL, Meerman JH, Commandeur JN, Vermeulen NP. (1999). Biomarkers of Free radical Damage Applications in Experimental Animals and Humans, Free Rad. Biol. Med. 26, 202-226., https://www.ncbi.nlm.nih.gov/pubmed/9890655

Donma O and Donma MM. ( 2017, November) Progranulin: Is It A New Adipocytokine at the Crossroads of Obesity, Metabolic Syndrome and Cancer?. LIFE: International Journal of Health and Life-Sciences, Volume 3 Issue 3,.29-37. https://dx.doi.org/10.20319/lijhls.2017.33.2937

Hamda Fauza, (2014). Gambier: Indonesia Leading Commodities in The Past, Int.J.on Advanced Science Engineering Information Technology, Vol.4 No. 6, 67-72. http://id.portalgaruda.org/index.php?ref=browse&mod=viewarticle&article=280118

Hanny Setyowati, Gambir (Uncaria gambir Roxb) as Natural Cosmeceutical Agent, CDK-250/ vol. 44 no. 3. 2017, 222-224. http://www.kalbemed.com/Portals/6/1_21_250Analisis-Gambir-ncaria%20gambir%20Roxb%20as%20Natural%20Cosmeceutical%20Agent.pdf.

Harborne JB. (1998). Phytochemical methods: A guide to modern techniques of plant analysis. 3rd ed. London: Academic Press, 192-204., https://books.google.co.id/books/about/Phytochemical_Methods_A_Guide_to_Modern.html?id=vCWHUU6iobwC&redir_esc=y

Hilal Y and Engelhardt U. (2007). Characterisation of white tea – Comparison to green and black tea, Journal Consumer Protection and Food Safety, 2, 414 – 421. https://www.scribd.com/document/248864247/White-Tea-Chemical-Analysis

Kasote DM, Katyare SS, Hegde MV, Bae H. (2015) Significance of Antioxidant Potential of Plants and its Relevance to Therapeutic Applications, Int. J. Biol. Sci. Vol. 11 (8): 982-991. doi: 10.7150/ijbs.12096. http://www.ijbs.com/v11p0982.pdf.

Kaushik AC and Sahi S, (2017, November) NMD Server: Natural medicines database for drug discovery, Life: International Journal of Health and Life-Sciences, Volume 3 Issue 2, 216-224. DOI- https://dx.doi.org/10.20319/lijhls.2017.32.216224.

Luo KW, Fung WF, Lung WY, Luo XL and Huang WR. (2017). Green Tea Polyphenol, Epigallocatechin-3-Gallate (EGCG): Mechanisms and Application on Hepatocellular Carcinoma. SM Liver J.; 2(1): 1004. file:///C:/Users/User/Downloads/fulltext_smlj-v2-1004.pdf.

Melton, SL. (1983). Methodology for following lipid oxidation in muscle foods. Food Technology ,37(7), 105- 109. http://agris.fao.org/agris-search/search.do?recordID=US8208627

Musdja MY (2012). Immunomodulatory Effect, Antibacterial Activity of Materials and the Mixture Materials of Betel Chewing and Comparative of Composition Between Betel Leaf Essential Oil with the Mixture Materials of Betel Chewing, Dissertation, Faculty of Medicine, University of Indonesia.

Musdja MY, Hapsari MA, Agusta A (2017). Comparison of Activity and Inhibitory Mechanism between (+)-Catechin and Water Extract of Gambier (Uncaria Gambir Roxb) Against Some Bacteria Scientific Journal of PPI-UKM, Vol. 4 No. 2, 55-60. http://www.kemalapublisher.com/index.php/ppi-ukm/article/view/300.

Northwest, Malondialdehyde Assay, Life Science Specialities, LLC, Premier Products for superior life Science Research. https://www.cellbiolabs.com/mda-malondialdehyde-assays-and-reagents.

Prior RL, Cao G. (2000). Antioxidant phytochemicals in fruits and vegetables: diet and health implications. Hortic Sci 2000;35:588–92.

Procházková D, Bousová I, Wilhelmová N. (2011). Antioxidant and prooxidant properties of flavonoids. Fitoterapia. 82(4):513-523. http://agri.ckcest.cn/ass/3ca91b10-107d-4bd7-bc38-3c6624c9e208.pdf.

Rangari VD. (2007. Tannin Containing Drugs, Pharmacognosy 1, 1187 - 198.

Sandhar HK, Kumar B, Prasher S, Tiwari P, Salhan M, Sharma P. (2011) A review of phytochemistry and pharmacology of flavonoids. Internationale Pharmaceutica Sciencia, 1(1):25-41

Sarafinovska ZA, Dimovski AJ. (2013). Natural antioxidants in cancer prevention, Macedonian pharmaceutical bulletin, 59 (1, 2) 3 – 14. file:///C:/Users/User/Downloads/NaturalAntioxidantsinCancerPrevention.pdf.

Södergren, E. (2000). Lipid Peroxidation in vivo Evaluation and Application of Methods for Measurement, Comprehensive Summaries of Uppsala Dissertations, Acta Universitatis Upsaliensis, Uppsala. http://uu.diva-portal.org/smash/record.jsf?pid=diva2%3A160811&dswid=5728

Standar Nasional Indonesia (National Standard Indonesia : SNI 01-3391-2000. http://teknologihutan.fkt.ugm.ac.id/userfiles/download/SNI_01-3391-2000_-_Gambir.pdf

Taniguchi S, Kuroda K, Yoshikado N, Doi KI, Tanabe M, Shibata T, Yoshida T, Hatano T. (2008). New Dimeric Flavans From Gambir, an Extract of Uncaria gambir. Japan: The Japan Institute of Heterocyclic Chemistry, Okayama University, 1-11 http://ousar.lib.okayama-u.ac.jp/files/public/3/34205/20160528031815252292/fulltext.pdf

Wills, ED. (1966). Mechanisms of Lipid Peroxide Formation in Animal Tissues, Biochem. J. 99, 667-676. https://www.ncbi.nlm.nih.gov/pubmed/5964963

Yagi, K. (1998). Simple Procedure for Specific Assay of Lipid Hydroperoxides in Serum or Plasma, Free Radical and Antioxidant Prot, 108, 101-106. https://www.ncbi.nlm.nih.gov/pubmed/9921520

Downloads

Published

2018-07-14

How to Cite

Musdja, M. Y., Rahman, H. A., & Hasan, D. (2018). ANTIOXIDANT ACTIVITY OF CATECHINS ISOLATE OF UNCARIA GAMBIER ROXB IN MALE RATS. LIFE: International Journal of Health and Life-Sciences, 4(2), 34–46. https://doi.org/10.20319/lijhls.2018.42.3446