• Ayu Saka W Laksmita Bali International University, Denpasar, Indonesia
  • Ni Putu Widayanti Bali International University, Denpasar, Indonesia
  • Desak Putu Risky Vidika Apriyanthi Bali International University, Denpasar, Indonesia
  • I G K Nyoman Arijana Department of Histology Faculty of Medicine Udayana University, Denpasar, Indonesia




Long Non-Coding, lncRNA LY86-AS1, Diabetes, T2DM


Diabetes mellitus type 2 (T2DM) is the most common health problem found in almost all over the world. T2DM sufferers need long-term care and treatment to prolong life and improve their life quality. Long noncoding RNA (lncRNA) is a part of noncoding RNA that was recently created with various human diseases. This research aims to explore the analysis of lncRNA LY86-AS1 as a diagnostic marker for T2DM. This study is quantitative descriptive using simple random sampling technique. Blood samples were taken from 10 respondents of Munggu society. Extracted RNA was used to synthesize complementary DNA with Reverse Transcription Kit. Quantitative real-time PCR has doneby SYBR Green. PCR analysis was performed with quantitative analysis by conducting 10 samples. The result found was the decrease in lncRNA LY86-AS1 expression. From this it can be concluded the possibility of using lncRNA LY86-AS1 for detecting diagnostic markers of T2DM sufferers for Munggu society.


Abhishek, S., Ji-panjang , Hao., Xiu-fen, Liu., Dan-dan, Z., Om, P.P., Ying Gao., PengHui., Xin-xuan, Dai., Cheng-wei, L. (2019). NONRATT021972 Long-Noncoding RNA: APromising lncRNA in Diabetes-Related Diseases.Int. J. Med. Sci, 16(6), 902-908. https://doi.org/10.7150/ijms.34200

Agilent Technologies. (2012). Introduction to Quantitative PCR: Methods and Applications Guide. IN70200 D.

Billings, L. K., Jose, C. F. (2010). The genetics of type 2 diabetes: what have we learned from GWAS?.Ann N Y Acad Sci., 1212: 59–77. Institutes National of Health. https://doi.org/10.1111/j.1749-6632.2010.05838.x

Giroud M, Scheideler, M.,(2017). Long Non-Coding RNAs in Metabolic Organs and Energy Homeostasis. Int J Mol Sci 18(12), 2578.

González A, et al. (2009). Normal Function of The Yeast TOR Pathway Requires The Type 2C ProteinPhosphatase Ptc1. Mol Cell Biol, 29(10). 2876-88. https://doi.org/10.1128/MCB.01740-08

Kaneto, H., Matsuoka, T. (2015). Role of Pancreatic Transcription Factors in Maintenance of Mature β-Cell Function. International Journal of Molecular Sciences. 16, 6281-6297; https://doi.org/10.3390/ijms16036281

Kurniawan. (2010). Diabetes Melitus Tipe 2 pada Usia Lanjut. Majalah Kedokteran Indonesia. 60 (12), 576-84.

Kong, X., Xing, X., Hong, J., Zhang, X., Yang, W. (2017). Association of A Type 2 Diabetes Genetic Risk Scorewith Insulin Secretion Modulated by Insulin Sensitivity Among Chinese Hans. Clin Genet. https://doi.org/10.1111/cge.12817

Kusnadi, G., Etisa, A. M., Deny, Y.F. (2017). Faktor Risiko Diabetes Melitus pada Petani dan Buruh. Journal of Nitrition College, 6(2), pp, 138-148. https://doi.org/10.14710/jnc.v6i2.16905

Lappin, S., Gomez, M., Knorr, D. (2012). Complete Automation of the Stratagene StrataPrep 96 PCR Purification Kit with the Agilent Bravo Automated Liquid Handling Platform and Agilent Automated Centrifuge. Agilent Technologies. Inc. Santa Clara. CA USA.

Lyssenko, V., Laakso, M. (2013). Genetic Screening for The Risk of Type 2 Diabetes: Worthless or Valuable?. Diabetes Care 36, (Supplement 2), S120–S126. https://doi.org/10.2337/dcS13-2009

Mansoori, Z., Hamid, G., Mirsaber, S., Rouhollah, V., Ali, R., Mehrnoosh S., Leyla, S., Faranak K. (2018). Downregulation of Long Non-Coding RNAsLINC00523 and LINC00994 in Type 2 Diabetes in an Iranian Cohort. Molecular Biology Reports, (45)5, pp, 1227-1233. https://doi.org/10.1007/s11033-018-4276-7

Mao, A.P., Shen, J., Zuo, Z. (2015). Expession and Regulation of Long Noncoding RNAs in TLR4 Signalingin Mouse Macrophages. BMC Genomics, 16, 45. https://doi.org/10.1186/s12864-015-1270-5

Nugroho , W. (2009). Komunikasi dalam Keperawatan Gerontik. Jakarta: EGC.

Olokoba, A., B., Olusegun, A., O., Lateefat, B., O., (2012). Type 2 Diabetes Mellitus: A Review of Current Trends. Oman Medical, 27(4), 269-273. https://doi.org/10.5001/omj.2012.68

Pastori C, Wahlestedt C. (2012). Involvement of Long Noncoding RNAs in Diseases Affecting the Central Nervous System. RNA Biol 9, 860-70. https://doi.org/10.4161/rna.20482

Robertson, R., P.(2004). Chronic OxidativeStress as a Central Mechanism for Glucose Toxicity inPancreatic IsletBeta Cells in Diabetes. JBC, Vol. 279,No. 41, pp, 42351–42354. https://doi.org/10.1074/jbc.R400019200

Saedi, L., Hamid, G., Mirsaber, S., Rouhollah, V., Ali, R., Mehrnoosh, S., Zahra, M., Faranak, K. (2018). Long non coding RNA LY86-AS1 and HCG27_201 Expression in Type 2 Diabetes Mellitus. Mol BiolRep. Springer Netherlands, 44(6), 2601-2608. https://doi.org/10.1007/s11033-018-4429-8

Sankhari, J. (2019). Effectiveness of Cassia Auriculata Flower (Avaram poo) Extract in Reducing Blood Glucose Among Pre Diagnosed Type 2 Diabetes Mellitus Clients in Selected Area of Puducherry, India. LIFE: International Journal of Health and Life-Sciences, 5(1), pp, 126-136. https://doi.org/10.20319/lijhls.2019.51.126136

Schmittgen, T. D., Livak, K. J. (2008). Analyzing Real-Time PCR Data by The Comparative C(T) Method.Nat Protocols, 3(6), 1101-1108. https://doi.org/10.1038/nprot.2008.73

Senevirathne, C. P., Katulanda, P., Dhanapala, N. D. (2015). The Prevalence of Hypertension, Obesity, Diabetes and Job Satisfaction among Public Health Workers in Central Province, Sri Lanka, LIFE: International Journal of Health and Life-Sciences, 1(2), pp, 25-47 https://doi.org/10.20319/lijhls.2015.12.2547

Sun X, Wong D. (2016). Long non-coding RNA-mediated regulation of glucose homeostasis and diabetes. Am J Cardiovasc Dis, 6(2), 17.

Sun, M., Kraus W., L. (2013). Minireview: Long Noncoding Rnas: New "Links" Between Gene Expression and Cellular Outcomes in Endocrinology. Mol Endocrinol, 27, 1390-402. https://doi.org/10.1210/me.2013-1113

Takahashi, K., Yan, I., Haga, H., Patel, T. (2014) Long noncoding RNA in Liver Diseases. Hepatology, 60: 744-53. https://doi.org/10.1002/hep.27043

Yuan, J., S., Reed, A., Chen, F.,Stewart, Jr., C., N. (2006). Statistical Analysis of Real- Time PCR Data. BMC Bioinformatics, 7, 1471-2105. https://doi.org/10.1186/1471-2105-7-85




How to Cite

Laksmita, W. A. S., Widayanti, N. P., Apriyanthi, D. P. R. V., & Arijana, I. G. K. N. (2020). LNCRNA LY86-AS1 EXPRESSION ON T2DM AGED 40 YEARS OVER IN MUNGGU SOCIETY. LIFE: International Journal of Health and Life-Sciences, 6(1), 73–82. https://doi.org/10.20319/lijhls.2020.61.7382