SHORT TERM INDUCTION OF LPS DAMAGES THE HIPPOCAMPUS GYRUS THROUGH APOPTOSIS ON GLIAL CELLS MICE

Authors

  • Shobihatus Syifak Department of Neurology, Faculty of Medicine, University of Nahdlatul Ulama Surabaya, Indonesia
  • Dyah Yuniati Department of Neurology, Faculty of Medicine, University of Nahdlatul Ulama Surabaya, Indonesia
  • Hidayatullah Department of Neurology, Faculty of Medicine, University of Nahdlatul Ulama Surabaya, Indonesia
  • Hotimah Masdan Salim Department of Biochemistry Medicine, Faculty of Medicine, University of Nahdlatul Ulama Surabaya, Indonesia

DOI:

https://doi.org/10.20319/lijhls.2021.71.0109

Keywords:

LPS, Sepsis, Hippocampus, Microglia, Inflammation

Abstract

Sepsis is a condition that causes the highest mortality in the ICU. Sepsis occurs due to an infectious process that causes damage to organs such as the brain. During the sepsis process, the brain plays a full role in the body's defense, so that it manifests as encephalopathy. However, the mechanism in short-term sepsis not fully describes. This study aims to examine brain damage to the hippocampal gyrus in experimental animals induced by LPS in a short time. Methods: Mus musculus mice aged 8 weeks were divided into three groups namely control group (Ctrl), induction of LPS for 4 h (LPS-4h), and induction of LPS for 8 h (LPS-8h). LPS was injected by intraperitoneal with 25 mg/kg. The brain damage was seen histologically with HE staining. Results: The results of LPS injection showed an insignificant increase in leucocytes among all groups.  Whereas the histological analysis found that glial cell damages increased significantly (P<0.05) with time dependency after LPS induction compared with the control group. Conclusion: Short-term induction of LPS destroys the hippocampal gyrus via high glial cells.

References

Angus, D. C., & Van der Poll, T. (2013). Severe sepsis and septic shock. N Engl J Med, 369, 840–851.https://doi.org/10.1056/NEJMra1208623

Frost, J. L., & Schafer, D. P. (2016). Microglia: architects of the developing nervous system. Trends in Cell Biology, 26(8), 587–597.https://doi.org/10.1016/j.tcb.2016.02.006

Garofalo, A. M., Lorente-Ros, M., Goncalvez, G., Carriedo, D., Ballén-Barragán, A., Villar-Fernández, A., Peñuelas, Ó., Herrero, R., Granados-Carreño, R., & Lorente, J. A. (2019). Histopathological changes of organ dysfunction in sepsis. Intensive Care Medicine Experimental, 7(1), 45.https://doi.org/10.1186/s40635-019-0236-3

González, H., Elgueta, D., Montoya, A., & Pacheco, R. (2014). Neuroimmune regulation of microglial activity involved in neuroinflammation and neurodegenerative diseases. Journal of Neuroimmunology, 274(1–2), 1–13.https://doi.org/10.1016/j.jneuroim.2014.07.012

Gotts, J. E., & Matthay, M. A. (2016). Sepsis: pathophysiology and clinical management. Bmj, 353.https://doi.org/10.1136/bmj.i1585

Hofman, A., Murad, S. D., van Duijn, C. M., Franco, O. H., Goedegebure, A., Ikram, M. A., Klaver, C. C. W., Nijsten, T. E. C., Peeters, R. P., & Stricker, B. H. C. (2013). The Rotterdam Study: 2014 objectives and design update. European Journal of Epidemiology, 28(11), 889–926.https://doi.org/10.1007/s10654-013-9866-z

Lee, B., Shim, I., & Lee, H. (2018). Gypenosides attenuate lipopolysaccharide-induced neuroinflammation and memory impairment in rats. Evidence-Based Complementary and Alternative Medicine, 2018.https://doi.org/10.1155/2018/4183670

Levy, M. M., Dellinger, R. P., Townsend, S. R., Linde-Zwirble, W. T., Marshall, J. C., Bion, J., Schorr, C., Artigas, A., Ramsay, G., & Beale, R. (2010). The Surviving Sepsis Campaign: results of an international Guideline-Based Performance Improvement Program Targeting Severe Sepsis. Intensive Care Medicine, 36(2), 222–231.https://doi.org/10.1007/s00134-009-1738-3

Negi, N., & Das, B. K. (2018). CNS: Not an Immunoprivilaged site anymore but a Virtual Secondary Lymphoid Organ. International Reviews of Immunology, 37(1), 57–68.https://doi.org/10.1080/08830185.2017.1357719

Parkhurst, C. N., Yang, G., Ninan, I., Savas, J. N., Yates III, J. R., Lafaille, J. J., Hempstead, B. L., Littman, D. R., & Gan, W.-B. (2013). Microglia promote learning-dependent synapse formation through brain-derived neurotrophic factor. Cell, 155(7), 1596–1609.https://doi.org/10.1016/j.cell.2013.11.030

Raymond, S. L., Holden, D. C., Mira, J. C., Stortz, J. A., Loftus, T. J., Mohr, A. M., Moldawer, L. L., Moore, F. A., Larson, S. D., & Efron, P. A. (2017). Microbial recognition and danger signals in sepsis and trauma. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1863(10), 2564–2573.https://doi.org/10.1016/j.bbadis.2017.01.013

Singer, M., Deutschman, C. S., Seymour, C. W., Shankar-Hari, M., Annane, D., Bauer, M., Bellomo, R., Bernard, G. R., Chiche, J.-D., & Coopersmith, C. M. (2016). The third international consensus definitions for sepsis and septic shock (Sepsis-3). Jama, 315(8), 801–810.https://doi.org/10.1001/jama.2016.0287

Sonneville, R., Verdonk, F., Rauturier, C., Klein, I. F., Wolff, M., Annane, D., Chretien, F., & Sharshar, T. (2013). Understanding brain dysfunction in sepsis. Annals of Intensive Care, 3(1), 15.https://doi.org/10.1186/2110-5820-3-15

Steckert, A. V, Comim, C. M., Igna, D. M. D., Dominguini, D., Mendonça, B. P., Ornell, F., Colpo, G. D., Gubert, C., Kapczinski, F., & Barichello, T. (2015). Effects of sodium butyrate on aversive memory in rats submitted to sepsis. Neuroscience Letters, 595, 134–138.https://doi.org/10.1016/j.neulet.2015.04.019

Yates, D. T., Löest, C. A., Ross, T. T., Hallford, D. M., Carter, B. H., & Limesand, S. W. (2011). Effects of bacterial lipopolysaccharide injection on white blood cell counts, hematological variables, and serum glucose, insulin, and cortisol concentrations in ewes fed low-or high-protein diets. Journal of Animal Science, 89(12), 4286–4293.https://doi.org/10.2527/jas.2011-3969

Yokoo, H., Chiba, S., Tomita, K., Takashina, M., Sagara, H., Yagisita, S., Takano, Y., & Hattori, Y. (2012). Neurodegenerative Evidence in Mice Brains with Cecal Ligation and Puncture-Induced Sepsis: Preventive Effect of the Free Radical Scavenger Edaravone. PLoS One, 7(12), e51539.https://doi.org/10.1371/journal.pone.0051539

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Published

2021-03-15

How to Cite

Syifak, S., Yuniati, D., Hidayatullah, ., & Salim, H. M. (2021). SHORT TERM INDUCTION OF LPS DAMAGES THE HIPPOCAMPUS GYRUS THROUGH APOPTOSIS ON GLIAL CELLS MICE. LIFE: International Journal of Health and Life-Sciences, 7(1), 01–09. https://doi.org/10.20319/lijhls.2021.71.0109